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KMID : 0370019980120000045
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Chung-Ang Journal of Pharmacal Sciences
1998 Volume.12 No. 0 p.45 ~ p.54
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Effect of Ca^2+and Mn^2+ ions to the activity of SBA lectin
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Abstract
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Soybean agglutinin (SBA) is the Ca^2+, Mn^2+ dependent lectin and the specificity of carbohydrates are D-galactose and N-acetyl-D-galactosamine. However, the effect of Ca^2+ and Mn^2+ ions to the activity of SBA is still unknown in detail. In the present study, hemmaglutination assay and enxyme-linked lectin assay (ELLA) have been applied. First, the carbohydrate-binding specificity of the crude SBA extract was examined, and D-galactose and N-acetyl-D-galactosamine exhibited the activity of the inhibition. The effect of Ca^2+ and Mn^2+ on the activity of SBA was observed by increasing the concentration of the ions, 1 to 50mM. SBA revealed the maximum activity when it contains 2mM Ca^2+ and/or Mn^2+ ion. Using these results, to isolate the SBA from crude extract, GaINAc-affinity column was applied containing Ca^2+ and Mn^2+ ions, and then eluted by same buffer without Ca^2+ and Mn^2+. SBA lectin which has hemagglutination activity was successfully isolated using its Ca^2+ and Mn^2+ dependent properties.
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